Product overview :
Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized. Propidium Iodide (PI) (MW=668.4 Da) is an intercalating agent and a fluorescent molecule which is membrane impermeant and generally excluded from viable cells. Upon entering cells, PI will bind to DNA and RNA by intercalating between bases. Once bound to the nucleic acids, its fluorescence is enhanced 20- to 30-fold. Ex_max= 536nm / Em_max= 617 nm. This kit provides a rapid and convenient assay for cell cycle and cell proliferation. For normal cells, the content of DNA is changed with the process of cell cycle. Observed DNA stained by dyes using flow cytometry to calculate percentage of G0/G1, S, and G2/M. It will be clear known that how about the distribution of cell cycle and the activity of proliferation. For apoptotic cells, DNAs in cells is degraded by endogenous nuclease activated and diffuse out of cells with the process of apoptosis. A highly definable sub-G1 peak occurs and is easily quantified by dyes. The change of DNA in apoptotic cells is also assayed for sorting and further analyzing apoptotic cells. After RNA is degraded by RNase, the nucleic acid dye in this kit bind with DNA composed of chromatin in the nucleus. And the results can be analyzed by flow cytometry. 

Storage condition :
This kit remains stable for at least one year if stored at -20°C and protected from light.

For Research Use Only. Not For Use In Diagnostic Procedures.